Effect of CO₂ and N₂ Gas on Insecticide Penetration and Transport in Insect Body

Abstract

The effect of CO₂ and N₂ gas on the insecticide penetration and transport in the adults of adzuki bean weevil, Callosobruchus chinensis L., was examined. The insects were dipped into an aqueous solution of various concentrations of insecticide, and then the solution remained on the surface of the insect body was removed. These insects were treated with saturated CO₂ and N₂ gas in incubated glass tube for one to sixteen hours respectively, and transferred into petri dishes. In the meantime, the insects recovered from narcosis, moved violently and at last died. The results varied depending on concentration of the insecticides and the exposed periods to each gas, and the higher the concentration of insecticide was, the less the moving activity and the shorter the survival periods were. The gas treatment also showed the same tendency.