Embryonic Tissue Culture of Laodelphax striatellus FALLÉN

Abstract

Embryonic tissues of the smaller brown planthopper, Laodelphax striatellus FALLÉN were cultivated for over 100 days in vitro. When the basic medium (Mitsuhashi's NCM-4A) was supplemented with the haemolymph of Philosamia cynthia PRYERI, active cell migration and mitoses were observed. Tissues either with or without trypsinization attached themselves well to the glass surface of culture vessels. In the trypsinized tissue cells migrated more actively than in the tissue without trypsinization. They continued growing to form epithelial cell sheets. Mitoses was observed in those cell sheets. There were many hollow spherical vesicles on the explant and the vesicles continued to swell up. In the embryonic tissue culture of L. striatellus, one type of fibroblast like cells, two types of wandering cells and three types of epithelial-like cells were observed. By the subculture of the original explants, including the growing tissues and the hollow spherical vesicles, similar vesicles were formed again on the explants, and active growing cells migrated from the original explants. However no cell growth was obtained from the separated cells.