Abstract
Effect of high temperature on the multiplication of a densonucleosis virus (DNV) in Bombyx mori was studied using the single radial immunodiffusion method for the virus titration. The multiplication of DNV was greatly reduced when the infected larvae reared at 25-28°C were transferred to an environment at the high temperature of 37°C. Autoradiographic results revealed that the synthesis of both viral DNA and protein was greatly reduced in the infected larvae maintained at 37°C. Fluorescent antibody studies also confirmed that the synthesis of DNV-antigen in the larva was inhibited at 37°C. These results indicated that high temperature (37°C) may reduce the activity of enzymes concerned with viral DNA and protein syntheses.
