On the Insecticidal Principle of Tricholoma muscarium KAWAMURA
I. Separative Experiment of the Insecticidal Principle and Free Amino Acids
1959 Volume 3 Issue 1 Pages 41-43
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1959 Volume 3 Issue 1 Pages 41-43
Tricholoma muscarium KAWAMURA has been used as a fly catcher not only in farmers but also in store houses in Tohoku districts. On the other hand it is of interest that this mash-room is edible.
An attempt was made to extract the insecticidal principle contained in this mushroom.
This principle is easily soluble in water, not in organic solvents, and is absorbed by Amberlite IR 120 (H type) from its water solution together with amino acids. The absorbed principle can be eluted with aqueous 1% NH4OH.
Two dimension paper chromatography (phenol: H2O, 4:1 added with 0.04% oxine, n-BuOH: AcOH H2O, 4:1:1v/v) was employed to detect amino acids contained in the concentrate of the elute.
Aspartic acid, glutamic acid, alanine, valine, glycine, serine and threonine were detected, and one spot seemed to be glucosamine or glutamine. Another 9 spots were developed, but unable to be determined.
