Abstract
Six entomopathogenic fungi (Beauveria bassiana (serotype B1), Beauveria brongniartii, Paecilomyces fumosoroseus, Paecilomyces lilacinus and Nomuraea rileyi) were grown in L-broth. Antigens were extracted from acetone-dried cells with 1/15M phosphate buffer, pH 7.0, at 130°C for 5hr. Crude polysaccharides of B. bassiana (serotype B1), P. fumosoroseus, and P. lilacinus, respectively, were extracted obtained by ethanol precipitation and purified by gel filtration on Sephadex G-100. A single peak of polysaccharides was obtained from each fungus and this fraction was further separated into 2 fractions (Fr. 1 and Fr. 2) by DEAE-cellulose column chromatography. In the agargel diffusion test, Fractions 1 and 2 of B. bassiana (serotype B1) or P. fumosoroseus gave a single precipitin line against the anti B. bassiana (serotype B1) serum. However, Fractions 1 and 2 of P. lilacinus did not react with the anti B. bassiana (serotype B1) serum. In the agar-gel diffusion test, polysaccharides obtained from B. bassiana (serotype B1), B. brongniartii, P. fumosoroseus or P. farinosus gave a single precipitin line against the anti B. bassiana (serotype B1) serum. On the other hand, polysaccharides obtained from N. rileyi or P. lilacinus did not react with the anti B. bassiana (serotype B1) serum. Accordingly, the six fungi were classified into two groups: B. bassiana-B. brongniartii-P. fumosoroseus-P. farinosus group and P. lilacinus-N-rileyi group.
