1964 Volume 8 Issue 3 Pages 185-190
An improved method for eliminating the effect of variance in body weight on the result of the bioassay experiment of PCP for the ‘Dojo’ fish was presented in this paper.
1) A linear relation was obtained between the log body weight of ‘Dojo’ fish and the log lethal time in various concentrations of PCP.
2) The time at the average body weight of all fishes tested in the regression line mentioned above was considered as the median lethal time. The median lethal concentration derived from the relation between the median lethal time and log concentration coincided well with the result obtained by the usual median lethal concentration is evaluated at 24 hours after dipping.
3) The median lethal concentration determined from large numbers mentioned above also coincided completely with that determined from small numbers.