Karyotype analysis of Amphibian chromosomes as teaching materials in science teaching (2)—A simple method for chromosome preparations from corneal epithelium of the frogs.—

Abstract

A simple method for chromosome preparations was demonstrated using corneal cells ofthe frogs. 1) colchicine treatment (1 mg/ml) for 10~14 hours. 2) hypotonictreatment (distilled water) for 90 minutes. 3) vapor fixation (100% acetic acid) for 1 minute. 4) chromosome preparation (70% acetic acid) by squashed method.