Japanese Journal of Biological Education
Online ISSN : 2434-1916
Print ISSN : 0287-119X
RESEARCH PAPER
A Method of Rapid and Simple Preparation of DNA from Plant Tissue
M. Kusumoto, H. Anzai, N. Kusumoto
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Keywords: high school and general education course, liquid nitrogen, long chain DNA, rapid and simple method, teaching material
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1999 Volume 40 Issue 1 Pages 13-20

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Abstract

This study reported detailed protocol and hints of DNA extraction for teaching material in high school and general education course. In this method, long chain DNA was able to be extracted within 50 minutes at room temperature without cooledcentrifuge, incubation shaker, or liquid nitrogen. The efficiency of DNA extraction by this method was comparable with conventional methods(Kusanagi and Kusumoto 1976).

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© 1999 The Society of Biological Science Education of Japan
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