Simplified Window-Method to Observe the Development of Chick Embryos

Abstract

This report is intended to simplify the window method for observation of the development of chick embryos. The pointed end of the eggshell was cut off 3 cm in diameter. The open end was covered with transparent wrap film, such as polyethylen wrap. The film was fixed with a hold-ring. The other hold ring was set at the opposite end of the eggshell. Both hold rings were held together with four rubber bands. Main points of improvement were as follows: 1) the eggs with the window were incubated in the plastic box on the shaker at 60 rpm at 38°C The humidity was supplied from the water in the plastic box, and fresh air was introduced into the plastic box by an air pump. 2) About 4 ml of the albumen was discarded from the eggs the third day after incubation. Then the eggs were incubated at 50 rpm at 38°C for 15 more days. Using this simplified window method, about half of the eggs developed for 18 days, which was 3 days before hatching. Possible application of this method to elementary and high school biological education was discussed.