RESEARCH NOTE
Acute Effects of Ultraviolet B Irradiation on in vitro Cultured Human Lymphocytes: Application in a School of Medical Technology
2002 Volume 42 Issue 3 Pages 136-140
Details
2002 Volume 42 Issue 3 Pages 136-140
To assess the potential of the application of biologically acute effects of non-ionizing radiation in a school of medical technology, human lymphocytes were radiated in vitro by ultraviolet B (UV-B) at wavelengths from 280nm to 320nm. Survival ratios of radiated lymphocytes were examined with a trypan blue exclusion test. Lymphocytes were damaged by UV-B irradiation. At UV-B irradiant of 5 × 10−4 Jm−2 or 8 × 10−4 Jm−2 survival ratios were
approximately 50% and 70%, respectively (significant at P < 0.05). The survival ratio decreased exponentially with incremental increase of UV-B irradiation. This suggests that the one-hit-one-target model (which is a well-known model of biological effects of ionizing radiation) will be applicable to the explanation of acute effects of UV-B irradiation on the human lymphocytes. Addition of xanthine oxidase/xanthine dehydrogenase, which produces activated oxygen, had no significant effect on lymphocyte survival.
In conclusion, the irradiation experiment of UV-B on in vitro cultured human lymphocytes is an alternative to ionizing radiation for induction of biologically acute effects, and is suitable for laboratory practice in a school of medical technology.
