JOURNAL OF JAPANESE ASSOCIATION FOR DIETARY FIBER RESEARCH
Online ISSN : 2186-5108
Print ISSN : 1343-1994
Application of Cecal Fistula in Rats. The Daily Variation of Cecal Fermentation in Rats fed Fructooligosaccharides.
Atsutane OHTAMasako OHTSUKIMasao HIRAYAMATakashi ADACHI
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1998 Volume 1 Issue 2 Pages 23-30

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Abstract

Fructooligosaccharides (FOS) which are indigestible sugars have many beneficial effects. These effects were partially caused of organic acids produced by luminal fermentation. In this study, we observed the daily variation of cecal fermentation using cecal fistula rats. Five weeks old Sprague-Dawley rats were anesthetized and the fistula put on their cecum surgically. Rats with cecal fistula were divided into two groups (8 rats a group) and fed a control diet or 5% FOS containing diet between 10:00 and 16:00 (mealfeeding) for 6 days. On the third and sixth experimental days, cecal contents were collected every three hours for 24h. The pH and organic acids concentration of cecal contents were measured. On the three way ANOVA analysis, three main factors (FOS, sampling time, experimental days) affected on the cecal pH and lactate concentration. In conclusion, it is suggested that the daily variation on the cecal fermentation may arise in rats under specific dietary condition such as meal-feeding.

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© Japanese Association for Dietary Fiber Research
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