Abstract
A cytotoxic estimation method was developed for denture adhesives based on their elution properties.Their defined amounts were dialyzed(cut-off MW:12000 to 14000)against the culture media for 1 and 5 days.The dialysates containig eluates from the adhesives were subjected to a cell culture test using HeLa cells and their toxicities were estimated by the relative number of living cells to the control.When applied to different types of commercially available denture adhesives, all products except one brand showed significant cytotoxicities with the tendency to increase in an elution time-dependent manner and gum-type products appeared to be the most toxic.Several products acidified or alkalized the dialysates, while such pH changes scarcely influenced the cell growth.The present method proved to have high versatility for various denture adhesives that enables estimation of their cytotoxicities in identical conditions regardless of diversity in their types(powder, cream or gum).
