Relationship between Eluted Monomers from Dentin-adhesive Resin Systems and Cytotoxicity

Abstract

The relation between monomers eluted from resin systems and cytotoxicities was investigated by cell culture tests.L-cells in 3ml of Eagle's MEM supplemented with 10% FCS were cultured to confluent sheaths in a tissue culture dish.Primers, bonding agents and composite resins filled in glass tubes were immersed in the center of the tissue culture medium.After 24 hours cultivation, cytotoxicities were evaluated, and the amounts of monomers eluted into the medium were measured by high-performance liquid chromatography.The following results were obtained.The primers, uncured bonding agents and composite resins showed intensive cytotoxicity.However, the cytotoxicities of bonding agents and composite resins were decreased as the light illumination time increased.Adequately cured bonding agents and composite resins showed less or no cytotoxicity.However, small amounts of monomers were detected.L-929 cells were stimulated by HEMA and TEGDMA released from resins.