Abstract
We attempted to test protein molecules being translated by polysomal poly (A)+ m RNAs isolated from seizure-susceptible EL mouse brain. A significant lower translational activity was observed in both mice that experienced seizure (EL [s]) and those that did not (EL [ns]), compared to progenitor dd Y mice at 100 days of age. Anal ysis of translational products using a SDS-PAGE showed some quantitative differences in 28-and 100-day-old EL mice.Among 100-day-old mice, these proteins containing molecular weights of 23.5, 32.5 and 33.0kDa were not observed in EL [s] mice by analysis of two dimensional gel electrophoresis. The deficiencies of three proteins ma ybe associated with performed seizure-susceptibility due to toss-up stimulation approximately 40 times once a week. The data with regard to addition of exogenous poly (A) suggests that altered initiation during the translational process in protein synthesis takes place in EL mice compared with dd Y mice.
