Abstract
Single nodal segments and shoot tips from young trees of Davidia involucrata BAILL. were used to establish initial cultures. For bud break, the best results were obtained on Broad-leaved woody plant medium (BW) with 3.16μM of BAP (N6-benzyl-aminopurine) and 0.316 μM of NAA (α-naphthylacetic acid). Multiple shoots in subcultures were proliferated on BW medium with 5.62 μm of BAP and 0.316 μM of NAA. The longer shoots for rooting stock were obtained after seventh subculture. Isolated shoots were rooted by culturing them on half-strength BW medium containing 0.1 or 0.5%of activated charcoal, 3.16 or 10.0 μm of IBA (β-indolebutyric acid) and 1.0 μm of NAA. The rooting percentages were from 85.0 to 100.0%. The plantlets could be hardened and transplanted to pots.
