The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
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RAPD markers linked to the tomato mosaic virus resistance gene, Tm-1, in tomato
Taku OHMORIMinoru MURATAFusao MOTOYOSHI
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1995 Volume 70 Issue 2 Pages 179-184

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Abstract

Random amplified polymorphic DNA (RAPD) markers were screened in two nearly isogenic lines (NILs) of tomato, one of which carried Tm-1 gene conferring resistance to tomato mosaic virus (ToMV) and the other carried its susceptible allele. Among 1030 polymerase chain reaction (PCR) products generated by using 220 different 10-base oligonucleotide primers, 12 fragments were polymorphic between the NILs. Six markers arbitrarily chosen from these 12 fragments were examined whether they link to the Tm-1 in 125 BC1 plants. No recombinations were detected between the five markers and the Tm-1. The other one marker was also proved to link to the Tm-1, but their genetic distance was not determined due to some difficulty in distinguishing the RAPD band from the adjacent PCR products.

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© 1995 by The Genetics Society of Japan
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