1995 Volume 70 Issue 2 Pages 197-209
Homozygous stocks for the second or the third chromosome of Drosophila melanogaster with a single insertional plwB element were screened for high crossability with D. simulans. Reciprocal crosses between each of these stocks and D. simulans were made, and the insemination rate at two or three days was examined. From two cycles of screening of the original 575 stocks, one stock (# 687) which showed high insemination rate was selected and was backcrossed to a w strain to substitute the background. We obtained a stock which showed 10% insemination rate with D. simulans males (control was 0%). No stocks exhibiting a high crossability with D. simulans females were acquired. Revertant strains, from which the P element had been lost, were obtained from the backcrossed #687 stock. The insemination rates of 13 revertants to D. simulans males ranged from 1% to 33%. Seven of these 13 were not significantly different from the control line but were significantly different from the backcrossed #687 stock. It was concluded that the mutation showing high crossability with D. simulans males was caused by the P element transposition.