Analysis of mouse hemoglobin α-chain locus mutation induced by N-ethyl-N-nitrosourea

抄録

In a mouse specific-locus test in stem-cell spermatogonia of mice after treatment with N-ethyl-N-nitrosourea (ENU), a mutation at the hemoglobin α-chain locus was obtained. In an original mutant, the mutation was detected as a change in band density using cellulose acetate electrophoresis, e.g. the slow moving band was much higher than that of normal F₁ mice. Yet, migration of the mouse bands was similar. In isoelectric focusing, the migration of a band toward cathode was observed, indicating that the variant hemoglobin is caused by mutation at an α-globin chain.
This mutation was found to be heritable and viable in homozygous condition from a genetic test on the mutant, suggesting that this mutation would be due to a base-pair change. According to the usual nomenclature of mutation at the hemoglobin locus, the allele symbol of this mutation was tentatively designated as Hbac^c3.