Abstract
1. Experiments have been conducted on the follwing percutaneous infection subject on five volunteers (Number 1-5): depositing the water containing the infective larvae of A.d. on the back of the middle finger of their right hand, and water containing the infective larvae of N.a. on the same part of their left hand. Rate of invasion of the larvae was obtained through the skin, 24.5%-62.0% by A.d. and of 31.1%-74.5% by N.a.
In recovering worms using the anthelmintic treatment, the former rate was 0%-4.2% and the latter 8.6%-65.7%.
2. Measuring the length and the width of the infective larvae of N.a. soaked in human serum at 28°C for 96 hours, and comparing them with an unsoaked case, growth and transformation of the former could not be found. Examination of their resistance to artifical gastric juice, neither could the intensification of the resistance of the former be found. Administrating the soaked larvae into the stomach of 4 volunteers (Numbers 6-9), worms by the anthelmintic treatment were not obtained.
3. Placing the larvae of A.d. into the mucoid of the small intestine which had been extracted from a puppy, and keeping it at 28°C for 96 hours, then isolating the larvae from the small intestine, growth in length and width, or transformation could not be found. Larvae of A.d. was administered into the mouth of a puppy, and after 10 days, it was isolated from the small intestine. Transformation of the middle 3rd stage larvae was perceptible.
Administering the larvae into the stomach of a volunteers (No.10), symptoms of the so-called the Wakana disease could be found. Worms were obtained by using anthelmintic treatment. The recovering rate of the worms was 64%.
