Abstract
A simple and rapid method for the determination of PCB in human milk and blood using and Improved Essencial Oil Distillator has been developed. The analytical procedure is as follows: 5.0g of sample was put into a boiling flask and then 5ml of 5N-KOH solution and 50ml of refined water were added. The Improved Essencial Oil Distillator was connected with the above flask and distilled for 1hr or 2hrs. After distillation, the n-heptane layer was collected in a small test tube, then 0.1g of activated Florisil was added, stirred for 10sec. and used for gaschromatographic analysis.
Organochlorinated pesticide residues, which inhibited PCB analysis, were treated with alkaline degradation and Florisil adsorption, but PCB decreased only about 5% by this procedure. The recovery rates of PCB added to human milk and blood were 87-93% and 88-105%, res pectively. The comparison of the standard method with this method showed a good result. The merits of this distillation method were low cost, easy procedure in a short time and a good repeatability.
