Abstract
Respiration and enzyme activities of Geotrichum candidum showing filamentous phase in bulking sludge was examined in comparison with yeast-like phase.
1) RQ value of endogenous respiration in F phase cells for the early period was slightly greater than that of Y-like phase cells, but considerable differences were not observed between both phases. NaN3, CH2ICOOH strongly inhibited the endogenous respiration of F phase cells.
2) The dissolved oxygen (DO) consumption of G. candidum on various substrates was generally greater in Y-like phase cells than in F phase cells, and there was, in particular, a remarkable difference of DO consumption when the organism was grown in the medium added with amino acid such as glycine, alanine and arginine. As3+ and Hg2+ showed almost equal inhibition on pyruvate oxidation in both phase cells, but Cu2+, Pb2+, CN- and HCHO inhibited strongly the respiration of Y-like phase cells.
3) On enzymes of Leucine aminopeptidase, Alcohol dehydrogenase, G-6-P dehydrogenase and Glutathione reductase, except 5'-Nucleotidase, F phase cells showed high activities. Acid phosphatase (PMase) in cells of Y-like phase showed a marked lower activity than in F phase cells. However, Acid PMase in cell free extracts from Y-like phase showed opposite results.
4) Acid PMase was activated by the addition of Ca2+, Mg2+ and Ba2+, and it was noted that the activity was markedly accelerated by Pb2+ and Cu2+ Mo6+ and W6+ showed complete inhibition by the activity.