Search for spring quality regulating oxidative stress

Abstract

[Background]

Since balneotherapy are available for ameliorating symptoms of oxidative stress related diseases, hot springs have some effects for reduction of oxidative stress. In this study, we examined the effect of 10 types of spring quality upon oxidative stress by estimating the intensities of oxidative stress in vivo.

[Methods]

Oxidative stress-induced mice were bathed in ten kinds of hot spring quality at 25℃ and 41℃ for 10 minutes. After three times of bathing with 24 h intervals, effects of spring quality were estimated by the intensity of oxidative stress of each mouse, which were determined by in vivo imaging system.

[Results]

We found induction of HSP72 by hot spring waters, which might be responsible for suppression of oxidative stress. In addition, we identified that sulfur containing spring (hydrogen sulfide type) showed reduced oxidative stresses. On the contrary simple radioactive spring showed increased oxidative stresses.

[Conclusion]

Our results strongly suggest that the oxidative stress control effect of sulfur spring (hydrogen sulfide type) is caused by the interaction between hydrogen sulfide and HSP 72-induced antioxidant enzyme. In contrast, simple radioactive fountain can cause oxidative stress by radon-induced ROS.