One hundred and fifty one specimens of the Amur minnow Phoxinus lagowskii steindachneri were collected from the An’noro River, Assabu, southern Hokkaido, Japan, from 2007 to 2009. Previously recorded primarily from eastern Honshu Island in Japan, the subspecies has hitherto not been recorded from southern Hokkaido. Its occurrence in the An’noro River is most likely to have resulted from artificial introductions, together with Ayu (Plecoglossus altivelis altivelis) from Lake Biwa, from the mid 1980’s to 1995, since which time the former has become established.