First record of Pseudogobio esocinus eggs on a shingle beach along the northern coast of the North Basin of Lake Biwa

Abstract

Two egg population samples, G2901 (n = 27) and G2902 (n = 22), were collected from a shingle beach along the northern coast of the North Basin of Lake Biwa, Japan. Eight eggs were randomly selected from each sample and the mitochondrial 12S rRNA region was partially sequenced (177 bp). All 16 eggs shared a single haplotype and were identified as Pseudogobio esocinus based on phylogenetic analysis. To date, eggs of this species found in lake environments (including those of the lakes other than Lake Biwa) have been reported only from a single sandy gravel beach along the eastern coast of the North Basin of Lake Biwa. The present finding is the first record of P. esocinus eggs from a shingle beach.