Abstract
1) The ovulation of one-year-old gold fishes is accelerated by injection of anterior pituitary extracts obtained from frogs as well as domestic fowls.The extracts were made with 40% ethanol or 2% NaCl aqueous solution by the procedure which was adopted by JENSEN et al. ('39) and CHOW et al. ('42) for extracting FSH and ICSH fractions from mammalian pituitaries.
2) The substance extracted with 40% ethanol was separated into two fractions by 0.5 and 0.8 saturated solutions of ammonium sulphate . Each fraction, however, accelerated the ovulation of gold fishes . This seems to be attributable to the impureness of both fractions.
