Abstract
Technique for implantation of cannula into dorsal aorta of fish was applied successfully to the carp, Cyprinus carpio, with body weight ranging from 500 to 1, 000 g.Effects of anesthesia and surgical operation were also investigated with hematocrit value as a parameter.In the carp, it is unnecessary to secure the cannula with any kind of fixation.Implanted cannulae can be used for 10 days or more, and as far as hematocrit value is concerned, the fish should be allowed to rest for at least 2 days after being cannulated.A few hours after the operation, hematocrit value decreases drastically against the value measured at the time of operation and then gradually takes a stable level 2 days after the operation.Hematocrit value increases when the fish is anesthetized by immersion in a standing solution as well as by irrigation with an anesthetic solution.
