Early Development of the Laboratory-Reared Flounder, Pleuronichthys cornutus

Abstract

Fertilized eggs of Pleuronichthys cornutus were obtained by both artificial fertilization and natural spawning of laboratory-reared fish. The present paper describes in detail the early development of the fish and the rearing methods employed to provide basic information for mass production of this species. Eggs and sperm for artificial fertilization were obtained from adult fish caught in the Ariake Sound, Kyushu in November and December of 1984. Their maturation was successfully induced by intermuscular injection of pituitary homogenate of the silver carp, Hypophthalmichthys molitrix. Fertilized eggs were also obtained in 1985 by natural spawning of a broodstock kept in a tank for a year. Hatched larvae were fed successively with rotifers, Artemia nauplii and the harpacticoid copepod, Tigriopus japonicus and reared for 80 days. Ten thousand young fish of about 33mm TL were obtained in 1984 and 1985 with the survival rate of about 17%. Ten developmental stages were defined on the basis of the morphological characteristics: A) newly hatched to 4 day old larvae, 2.7 to 4.1mm TL (2.6 to 3.9mm NL), yolk sac present; B) 4 to 16 day old larvae, 3.8 to 5.9mm (3.6 to 5.6mm), yolk resorbed, actively feeding on rotifers; C) 15 to 30 day old larvae, 6.3 to 8.3mm (6.0 to 7.9mm), notochord straight, hypural fin ray visible; D) 24 to 40 day old larvae, 6.7 to 9.2mm (6.4 to 8.8mm), caudal notochord upturned (45°); E) 28 to 45 day old larvae, 7.9 to 10.8mm (7.5 to 10.3mm), caudal notochord upturned (45°-90°); F) 32 to 50 day old larvae, 10.8 to 15.7mm (8.8 to 12.8mm BL), eyes symmetrical; G) 35 to 66 day old larvae, 13.4 to 20.0mm (10.9 to 16.3mm), eyes asymmetrical, but left eye not visible from the right side; H) 40 to 75 day old larvae, 13.8 to 26.2mm (11.3 to 21.4mm), the upper edge of left eye visible over top of the head from the right side; I) 46 to 89 day old larvae, 20.1 to 27.4mm (16.4 to 22.4mm), left eye on the edge of the head and pupil visible from the right side; and J) juveniles of 51 day old or over, 23.6mm or more (19.3mm or more), metamorphosis completed. One to three inflections were found for relative growth of total length, eye diameter, upper jaw length, preanal length, and distance between the base of the pectoral fin and the anus against the notochord length or body length. Two inflections were found for body length (or notochord length)-body weight relationship.Most inflections appeared at the stages of D, F and J, corresponding to the body length of 8, 9-12 and 18-22mm respectively.