仁淀川および物部川水系におけるDNA多型によるギンブナのクローン判別とそれらの分布

抄録

Silver crucian carp, Carassius langsdorfii, a triploid gynogenetic fish were collected from the Niyodo and Monobe Rivers, Kochi prefecture, Japan.Clonal lines were identified by genetic markers such as isozyme patterns (PROT, CK, PGM, GPI, EST), DNA fingerprinting and RFLP of the D-loop region of mtDNA. A total of 17 clonal lines were detected by DNA fingerprinting and isozyme genotypes. Six clones were common to both rivers, with ten and one being restricted to the Niyodoand Monobe Rivers, respectively. In RFLP analyses of the D-loop regionofmtDNA, eight haplotypes were detected, one to the four clones beingincluded ineach. Average haplotypic diversity (h) among the clonal lines wasrelatively hig (0.782) in RFLP, whereas the average inter-clonal BSI was low (0.195) in DNA fingerprinting. The factor responsible for the high geneticdivergence among the 17 silver crucian carp clones was discussed from the view points of their clonal nature and gynogenetic reproduction.