Abstract
A strain of Sporothrix schenckii (named KONODAI strain) happened to be isolated from scales in the eczematous lesion on a woman's buttock without clinical features characteristic of sporotrichosis. The colony of the isolated fungus on Sabouraud's dextrose agar was slightly moist with pigmentation, and the development of pigmentation was more evident on corn meal agar. The fungus produced delicate septate hyphae that gave rise to short conidiophores bearing a cluster of conidia. Conidia were also formed along the sides of hyphae. The fungus converted to a yeast-phase of growth on brain heart infusion dextrose agar at 37°C. It required thiamine for growth. Mice that received intraperitoneal injection of its yeast-phase cells developed peritoneal and peritesticular lesions. Furthermore it was brightly stained with fluoresein-labeled anti-S. schenckii rabbit globulin.
It was identified as S. schenckii for the reasons mentioned above.
There had been some reports on the isolation of S. schenckii from natural sources such as soil, plant debris or wood, but the isolation of the fungus from nonsporotrichotic human skin surface or scrapings of scales was uncommon.
