Comparative Studies on the Postantifungal Effect of Cispentacin and Pradimicin (BMY-28864) on Candida albicans

Abstract

The in vitro postantifungal effect (PAFE) following exposure of Candia albicans to cispentacin and pradimicin (BMY-28864) for short periods of time was investigated. A turbidometric method was used to measure cell regrowth following exposure to different concentrations (0.313 to 20μ/ml) of the two drugs for 0.5, 1 and 2hr at 30°C and 37°C. The PAFE was determined by the difference in time (hr) required for growth of the control and test cultures to reach the 0.5 absorbance level after removal of the drug by dilution. The length of the PAFE was dependent upon the drug concentration, exposure time and temperature and the strain of C. albicans being assayed. Optimal PAFEs ranged from 3.7 to >17.0hr with cispentacin and from 1.7 to >21.0hr with pradimicin (BMY-28864).