Nematological Research (Japanese Journal of Nematology)
Online ISSN : 1882-3408
Print ISSN : 0919-6765
ISSN-L : 0919-6765
A rapid method of DNA detection of the beet cyst nematode, Heterodera schachtii, in infested field soil
Erika Asamizu Satoshi KitabayashiHideaki Iwahori
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2022 Volume 52 Issue 1_2 Pages 1-8


Infestation of vegetable fields by the beet cyst nematode, Heterodera schachtii, was first reported in Japan in 2017. After pesticide treatments, fields should be monitored for the possible re-establishment of the nematode population. This paper describes a rapid method of DNA detection by direct extraction of DNA from soil using saturated phosphate buffer, followed by real-time PCR using H. schachtii-specific primers. Accurate handling of many soil samples collected from large fields is possible; however, the method has a limited success rate of one second-stage juvenile (J2) or ten cysts per 10 g of soil, which was determined by adding J2s or cysts in vitro. Using this method on 141 soil samples collected from an infested field, a clear correlation between DNA level and the number of hatched J2s was found. This method enables rapid detection and semi-quantitative measurement of cyst nematodes in the soil.

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© 2022 The Japanese Nematological Society
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