1994 Volume 35 Issue 3 Article ID: jjom.H05-173
The nutritional requirements for the vegetative growth of Lyophyllum shimeji were investigated by use of a synthetic liquid medium. A wide range of carbohydrates served as carbon source in the medium which supported growth of L. shimeji. Glucose, xylose, mannose, fructose, sucrose, dextrin, glycogen, pectin and soluble starch were especially good carbon sources for mycelial growth. Meat extract, Soyton, yeast extract, polypeptone, casamino acids and the amino acids mixture of the basal medium were acceptable nitrogen sources for the growth, while ammonium and nitrate salts were poor nitrogen sources. The amino acids mixture in the culture medium could be replaced by l-alanine, l-isoleucine, l-valine, l-glutamine, l-citrulline or l-serine. The vegetative mycelium did not grow in the absence of KH2PO4 and MgSO4, and the yield of mycelium was decreased by the omission of ZnSO4, FeSO4, thiamine and nicotinamide. The vegetative growth was accelerated in the basal medium containing 0.1-1 mg/l of indole-3-acetic acid (IAA), kinetine, gibberellic acid, 1-naphthyl acetic acid (NAA) and 2,4-dichlorophenoxy acetic acid, but it was inhibited in the basal medium containing 10 mg/l of IAA and NAA.
