The nutritional requirements for the vegetative growth of Lyophyllum shimeji were investigated by use of a synthetic liquid medium. A wide range of carbohydrates served as carbon source in the medium which supported growth of L. shimeji. Glucose, xylose, mannose, fructose, sucrose, dextrin, glycogen, pectin and soluble starch were especially good carbon sources for mycelial growth. Meat extract, Soyton, yeast extract, polypeptone, casamino acids and the amino acids mixture of the basal medium were acceptable nitrogen sources for the growth, while ammonium and nitrate salts were poor nitrogen sources. The amino acids mixture in the culture medium could be replaced by l-alanine, l-isoleucine, l-valine, l-glutamine, l-citrulline or l-serine. The vegetative mycelium did not grow in the absence of KH₂PO₄ and MgSO₄, and the yield of mycelium was decreased by the omission of ZnSO₄, FeSO₄, thiamine and nicotinamide. The vegetative growth was accelerated in the basal medium containing 0.1-1 mg/l of indole-3-acetic acid (IAA), kinetine, gibberellic acid, 1-naphthyl acetic acid (NAA) and 2,4-dichlorophenoxy acetic acid, but it was inhibited in the basal medium containing 10 mg/l of IAA and NAA.

　Methylated saccharides from yeast cells were analysed by thin layer chromatography (TLC) using diatomaceous earth as a support. Residues from which fatty acids had been extracted for analysis of yeast cell constitution were used as samples. Freeze-dried yeast cells (50 mg) were hydrolysed with 5% methanolic hydrochloride acid, and fatty acid methyl ester was extracted from the hydrolate with n-hexane. Chloride ions were removed from the residual solution with ion exchange resin. The solution was concentrated and used as a sample. Thin layer plates were prepared with mixture of diatomaceous earth (Wako Pure Chemical Indus.) and plaster (9 : 1). A mixture of 67% isopropanol (35 ml) and ethyl acetate (65 ml) was used as the solvent for the one-step development. Anthrone-sulfuric acid reagent was used for detection. The Rf value (×100) of the light yellowish brown spot of xylose was 60. Mannose gave a dark brown major spot of Rf value 47 and a minor spot of Rf value 25, and glucose gave a bluish purple spot of Rf value 40. For galactose, two clear, light yellow spots (Rf value, 25 and 28) and two minor spots (Rf value, 45 and 55) were observed. The presence of minor spots indicating anomers of mannose and galactose did not disturb the detection of major spot of methylated saccharides in mixed solution. Mannose, glucose and galactose were detected in strains of Geotrichum, Kluyveromyces, Pichia and Schizosaccharomyces by this thin layer chromatography. Glucose and galactose were detected in the strain of Cryptococcus and Trichosporon, together with minor spots of xylose. The methylated saccharide mixture could not be separated on a thin layer plate prepared with Kieselgur G of Merck (Germany). This one-step TLC may be suitable to the case of a few samples whose constituent saccharides need to be known immediately, as it needs no multiple development.

Two taxa of Inocybe collected by Kumagusu Minakata were identified as I. glabrodisca and I. aff. dunensis, respectively, and are new to the Japanese mycoflora. Their microscopic features are reported with illustrations.

　Fruit-body formation of Lyophyllum shimeji was achieved on a solid medium. The solid medium was prepared in bags by adding 750 ml of liquid medium (recipe: soluble starch, 100 g; D-glucose, 25 g; pectin, 1 g; yeast extract, 3 g; KH₂PO₄, 0.5 g; MgSO₄・7H₂O, 0.5 g; thiamine-HCl, 1 mg; CaCO₃, 5 g; charcoal powder, 5 g; water, 860 ml) to 120 g of peat moss. Cultures were incubated in darkness for 90 days at 23°C, then under fluorescent lamp illumination (50 lux.) for 30 days at 16°C. Following transfer to light at 16°C, primordia appeared on the media after 13-15 days and grew into fruit-bodies after 14-17 days. All of 27 strains of L. shimeji formed fruit-bodies, and fruit-body yields were 14.6-62.7 g/bag.