1994 Volume 35 Issue 3 Article ID: jjom.H05-192
Fruit-body formation of Lyophyllum shimeji was achieved on a solid medium. The solid medium was prepared in bags by adding 750 ml of liquid medium (recipe: soluble starch, 100 g; D-glucose, 25 g; pectin, 1 g; yeast extract, 3 g; KH2PO4, 0.5 g; MgSO4・7H2O, 0.5 g; thiamine-HCl, 1 mg; CaCO3, 5 g; charcoal powder, 5 g; water, 860 ml) to 120 g of peat moss. Cultures were incubated in darkness for 90 days at 23°C, then under fluorescent lamp illumination (50 lux.) for 30 days at 16°C. Following transfer to light at 16°C, primordia appeared on the media after 13-15 days and grew into fruit-bodies after 14-17 days. All of 27 strains of L. shimeji formed fruit-bodies, and fruit-body yields were 14.6-62.7 g/bag.
