Functional Role of CD80/86 and CTLA-4 in Interaction between T Cells and Antigen Presenting Cells on Contact Hypersensitivity

Abstract

The functional expression of CD80 and CD86 and their ligands, CD28, and CTLA-4 in the interaction between T-cells and dermal antigen-presenting cells was investigated by using a hapteninduced murine contact hypersensitivity model. Administration of anti-CD86 monoclonal antibody (mAb), but not anti-CD80 mAb, at the time of sensitization efficiently inhibited ear swelling. Immunohistochemical staining revealed that the migrated LC in the dermis express CD86 but not CD80, Further administration of mAb against CTLA-4, which is supposed to transduce a negative signal to T-cells, resulted in enlargement of ear swelling. These data suggested that CD86 on migrated Lc may play an important role for the interaction with naive T-cells in the regional lymph nodes and CTLA-4 may also contribute to this reaction.