Experimental radiosensitizer therapy for malignant tumor transplanted to nude and conventional mice

(2) Radiosensitive effect of Dinitroimidazole ethanol for Ehrlich solid carcinoma

Abstract

Dinitroimidazole ethanol is a new radiosensitizer of hypoxic tumor cells.
The purpose of our study is to compare the sensitizer enhancement of misonidazole and dinitroimidazole ethanol.
Methods and evaluation: Battelle Columbus Laboratories Protocol.
Radiation: ⁶⁰Co 15 Gy local radiation. These radiosensitizers were administered into the mice's abdomen before 30 minutes of local irradiation ⁶⁰Co 15 Gy.
Tumor: Ehrlich solid carcinoma. On the first day of the experiment, 2-3 pieces of 1-3mm tumor blocks in Eagle's MEM medium were transplanted to subcutaneous region of the right thigh of each mouse.
Subjects: Male conventional DDY mice, conventional ICR mice and nude ICR mice.(10 weeks, about 20g) Results: The most effective case was DNIE 10 mg+⁶⁰Co 15 Gy in DDY mice and second case was DNIE 10mg+⁶⁰Co 15 Gy in conventional ICR mice. There was no significant differences in tumor growth among the control group, ⁶⁰Co 15 Gy group and DNIE 2mg+⁶⁰Co 15 Gy group with regard to Ehrlich solid carcinoma.