Abstract
We have found out the relationship of nanoemulsion containing nano vitamin C, E and propolis and gingival disease. We have confirmed effect of nanoemulsion through the experiment of in vivo and in vitro. We tested cell viability of gingivalfibroblast cells by MTT assay and m RNA appearance of interleukin-1 β, using mouse that was guided inflammation. Anti-microbacterial activity for Antibacterial effect's experiment was carried out by using S.aureus and E.coli. In addition, inflammation tissue has been observed with scanning electrical microscopy. In thisstudy, expression of interleukin-1βA was decreased after adding nanoemulsion containing nanovitamin C, E and propolis. We've also obtained good results from the test of Antibacterial effect against S.aureus and E.coli. Also, swelling of inflammation tissues observed by scanning electrical microscopy has gone down.
In conclusion, we have gained confidence that nanoemulsion containing nano vitamin C, E and propolis has very high Antibacterial effect against bacteria in oral. And it made us guess that inflammation of gingival reduces after decreasing interleukin-1 β.
Thus, we expect that nanoemulsion containing nano vitamin C, E and propolis gives good effects to patient having gingival disease.
