This study aims at clarifying the physiological relaxing effect which pink rose brings about for 55 high school students. The stimulation was made by 30 pink roses with non-scent arranged in a vase while the control had no flower for 4 minutes. The physiological indices were the heart rate variability and pulse rate which employed fingertip accelerated plethysmography, and the psychological indicators were the SD method and the shortened version of profile of mood states (POMS). The results as follow: (1) HF were significantly higher, and LF/HF were significantly lower, (2) "comfortable", "natural", and "relaxed" feelings and the mood state were significantly better, while viewing fresh rose flowers than control.