2018 年 44 巻 11 号 p. 582-586
A simple spectrophotometric assay was developed for quantitative determination of selenium in selenium injection which is a hospital preparation for patients with selenium deficiency. Aliquots of the samples (500 µL) added 20 µL of 2.5 M formic acid and 20 µL of 18.5 mM O-phenylene diamine were incubated for two hours at room temperature. The absorbance was measured in the mixture at 333 nm by using NanoDrop®, a convenient instrument for spectrophotometric assay. The standard curve for determining selenium concentration was linear in the range of 1.25-30 µg/mL (r > 0.999). Coefficients of variation for intra- and inter-day assays were 0.3 to 5.5% and 0.8 to 2.8%, respectively. Assay validation was confirmed by five independent measurements of 50 µg/mL selenium injection in three pharmacists with the accuracy of 0.0 to 1.6%. This assay method was successfully applied to the quantitative determination of selenium in selenium injection.