Japanese Journal of Hospital Pharmacy
Online ISSN : 2185-9477
Print ISSN : 0389-9098
ISSN-L : 0389-9098
Simple Test of Hydrocortisone in Powdered Preparations
TOMONORI KOYAMA, MITSUYOSHI OKAMOTO, AKIYOSHI FUTAMURA, SHIGEO GOTO, SEIGO TAMAOKI
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Keywords: hydrocortisone, thin layer chromatography, simple test, clinical applications on hospital pharmacy
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1986 Volume 12 Issue 1 Pages 37-40

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Abstract
A thin-layer chromatographic method for the determination of hydrocortisone in powdered preparations after the treatment of hydrocortisone with sulfuric acid is described. After developing with chloroform/methanol (85: 15 v/v), silica gel plates were dried, sprayed with sulfuric acid solution, and heated. Hydrocortisone spot on the chromatogram was measured with a TLC densitometer equipped with a dual-wave length TLC scanner at λEx 380nm, AEM 550nm.
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