Methylprednisolone (MP) jelly was prepared for local application for treatment of the proctitis, which was sometimes caused by the radiation therapy for cervical cancer of the uterus. The stability and the release of the MP from the MP jelly were investigated using the highperformance liquid chromatography (HPLC). The results were as follows:
1) The MP jelly was sufficiently stable at 4°C for 14 days after preparation, while at room temperature the amount of MP in the jelly was decreased to 74% after 14 days. 2) The 50% release of MP from MP jelly required 180 min under the stationary condition; however, under the stirring condition the 50% and 100% release required 45 min and 180 min, respectively.
This preparation showed a good clinical effect.

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Protein content in syrup preparation of commercial lysozyme chloride could not be determined by Lowry method or spectrophotometric measuring at 280 nm due to the presence of additives in syrup which interfered with the measurements. However, highly accurate protein content could be obtained by a filter paper technique for the microdetermination of protein based on Lowry method where protein content in the syrup obtained by this method corresponded to potency. Specific activity of lysozyme in the syrup determined by use of Micrococcus lysodeikticus in suspension as lysozyme substrate was the same activity as purified lysozyme. Therefore, it seems that lysozyme highly purified was used for the syrup preparation.
The compatibility of lysozyme syrup with some commercial syrup preparations was studied. When Inolin syrup was mixed with the lysozyme syrup, 50% of the lysozyme activity was lost after 7 days at room temperature, whereas significant loss was not observed at 4°C. However, other syrups showed good compatibility with the lysozyme syrup under the same condition. Consequently, lysozyme chloride syrup seemed to be a stable preparation and to be mixed with other syrups.

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Quality tests were made on two kinds of oleic acids as raw materials (OA), JIS extra pure reagent (OA-JIS) and guaranteed reagent with content of about 99%(OA-GR), which were used for the material of ethanolamine oleate injection (EO-i). Furthermore, the effect of the difference of OA on the quality of EO-i was studied.
The content of peroxide in OA-JIS was higher when it was stored at room temperature than in a cold place. Therefore, it is necessary to store OA-JIS in consideration not only of light and air but of temperature.
The pH of EO-i with OA-GR was same as that with OA-JIS, while the viscosity of EO-i with OA-GR was higher. EO-i with OA-GR remained colorless before and after sterilization, whereas in the case of OA-JIS, the pale yellow color became more intense by sterilization. Light and temperature had no effect on the stability of EO-i, e. g., production of peroxide, the content of oleic acid and pH.
Although the further comparative study on effectiveness and safety of OA-GR and OA-JIS as the sclerosing agent for esophageal varix is necessary, it is thought, from the above results, that the use of OA-GR is desirable in pharmaceutical aspect.

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Determination of corticoids in commercial preparations by micro high performance liquid chromatography (MHPLC) was studied. The analytical conditions were as follows: column, HP-01 (10μm, 0.5 mm i. d.×14cm, Teflon tube); flow rate, 16 or 8, μl/ min.; injection volume, lμl; volume of mobile phase for one assay, 50-25μl
MHPLC is considered to be a convenient method for the rapid analysis of a small amount of pharmaceutical preparations at hospital pharmacy.

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A simple method for measuring the volume of liquid absorbed by tablets was devised and applied to the liquid penetration test with commercialized tablets. In case of the penetration of ethyl acetoacetate, the swelling of tablets was not observed, and the V² vs. t plots showed good linearity where V is the volume of liquid taken up. expressed in μl. and t is the time in second. In case of the water penetration, tablets swelled remarkably and the V ² vs. t plots showed the concave lines, but the log V vs. log t plots showed a good linearity. The slopes of the regression lines obtained from the log V vs. log t plots were in the range of 0.5 to 1.0.

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The dissolution patterns of 2 kinds (A and B) of sustained-release preparations of the ophylline were measured with the J. P. dissolution tester in the first fluid and the second fluid of the J. P. disintegration test. In addition, the dissolution pattern of tablet half on breaking of scored tablet of sample B was measured in the second fluid. The following results were obtained.
1) In sample A, the times required for 50% dissolution in the first fluid and in the second fluid were about 8 hours and 10 hours, respectively.
2) In sample B, the times required for 50% dissolution in the first fluid and in the second fluid were about 7 hours and 4 hours, respectively.
The dissolution patterns of both samples were linear. As the dissolution patterns of tablet half, the time required for 100%dissolution was about 10 hours.

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A new fully-automatic dividing and packing machine model OMP-Z (Tokyo Shokai) for powder and granule drugs was tested for a) error of dividing weight, b) accuracy of dividing weight in relation to the physical properties of the drug to be divided and packed, c) accuracy of dividing weight in relation to the number of subpacks and the quantity in each subpack, d) the rate of loss, and e) contamination.
The mean coefficient of variation (C. V.) of 21 drugs was 2 to 3% for 14 subpacks of 1 g in theoretical weight. This variation was lower than those of the existing fully-automatic dividing and packing machines. The rate of loss ranged from 0 to 24.8%, and was 3% or less for most drugs. However, it increased with drugs which were very adhesive.
The results of the tests were analyzed in relation to the angle of repose and dustability of the drugs. Errors in dividing weight were correlated with the angle of repose. They tended to become greater with an increased angle of repose.
The accuracy of dividing weight was examined in relation to the number of subpacks and the quantity in each subpack. 14, 28 and 42 subpacks of 0.5, 1.0, or 2.0 g in theoretical weight were prepared from 6 drugs. In some of these drugs, errors in dividing weight increased with smaller dose in each subpack and a greater number of subpacks.
Contamination was tested as follows: Lactose stained with eosin was first divided and packed, followed by the dividing and packing of lactose without eosin staining. The content of eosin in the subpacks of lactose without eosin staining was measured by means of spectrophotometry. The mean content of eosin was 3.5%.
In summary, the machine will be practical in use if the features of the machine are fully understood although some points still remain to be improved.

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A thin-layer chromatographic method for the determination of hydrocortisone in powdered preparations after the treatment of hydrocortisone with sulfuric acid is described. After developing with chloroform/methanol (85: 15 ^v/_v), silica gel plates were dried, sprayed with sulfuric acid solution, and heated. Hydrocortisone spot on the chromatogram was measured with a TLC densitometer equipped with a dual-wave length TLC scanner at λEx 380nm, AEM 550nm.

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