Determination of Antiepileptic Drugs in Body Fluid by a Competitive Binding Enzyme Immunoassay and Clinical Application

Abstract

A competitive binding enzyme immunoassay (MARKIT) procedure for determination of the concentration of antiepileptic drugs was assessed and compared with gas-liquid chromatographic (GLC) procedures. Furthermore the Possibility of antiepileptic drugs determination in serum ultrafiltrate and mixed saliva was investigated with MARKIT. The two methods of MARKIT and GLC gave essentially the same results in determination of the serum levels of phenobarbital. The correlation coefficient of MARKIT between GLC was as high as 0.922. The recovery of the phenytoin in serum, serum ultrafiltrate and saliva were 98.1±1.9, 97.5±2.9 and 72.5±3.6%, respectively. The correlation coefficients between serum and serum ultrafiltrate and serum and saliva were 0.926 and 0.654, respectively. These data suggest that MARKIT is a reliable method for the determination of the concentration of antiepileptic drugs in serum, serum ultrafiltrate and saliva.