論文ID: 0504270001
This study investigated changes in nitric oxide (NO) together with inducible nitric oxide synthase (iNOS) content and enzyme activity at 0, 4, 12, 24, and 48 h following acute muscle stretch injury. A single stretch injury was induced to the tibialis anterior muscle of 30 male New Zealand white rabbits (n = 6 at each time point). Injured and uninjured contralateral sham-operated muscles were harvested and analyzed for NO levels, iNOS content, and iNOS activity at each time point. In addition, three animals were used to estimate baseline NO levels and iNOS activity. There was a progressive reduction in NO content in both the injured and sham-operated muscles up to 24 h post-operation and stretch injury (p < 0.05). At 48 h post-injury, however, NO levels were 146% higher in injured muscles than sham-operated muscles (p < 0.05). iNOS protein content was higher at 4 h and 48 h in injured versus sham-operated muscle (p < 0.05). Similarly, iNOS activity was higher both at 4 h (p < 0.05), and 48 h (p < 0.01) in injured versus sham-operated muscles. These results suggest that NO may play an active role during the post-injury recovery of skeletal muscle modulated by iNOS expression.
