Abstract
The centrifugal ultrafiltration method was proposed as means of estimating the extracellular fluid which is equilibrated with diffusible SCN in plasma. By using this method, diffusible SCN in plasma could be determined under more nearly physiological conditions regarding temperature, pH and concentrations of the sample. Over the SCN concentrations range 5 to 60 mg/dl in plasma, the ratios of the diffusible SCN to the total SCN were 0.798, 0.940 and 0.863 in human, dog and rabbit respectively. These values were considerably variable according to the temperature, pH and albumin content of plasma.
At the same time, the red cells were found to keep 27.9% of the total SCN when SCN was added to rabbit blood. Moreover, it was found by the same ultrafiltration of rabbit red cell hemolysate mixed with SCN that SCN in red blood cells is not entirely dissolved in red cell water but two-fifths of it is the non-diffusible state.
