Abstract
1. Effects of some chemical agents upon the EIRG (electro-intraretinogram) of the bullfrog's eye were examined with the micro-pipette inserted into different retinal layers.
2. PIII, which was isolated by potassium from the other components in the ERG, was found to reverse its polarity to make the mirror image, when the pipette was inserted into a retinal layer external to the bipolar cells. This finding was taken as the proof that PIII or at least major part of it is originated in the bipolar cell layer.
3. Disappearance of the d-wave by alcohol or of the b-wave by adrenalin was found to follow extinction of the relating intraretinal negative potential, supporting the view that one part of the d-wave has its origin similar to the b-wave.
4. The possibility, that the negative potential in the EIRG might be the mirror image of the b-wave due to placing the micro-pipette at the opposite side of the doublet layer responsible for the generation of b-wave, appeared improbable, since it was found that acetylcholine removes the negative potentials while the b-and d-waves still exist in sufficient magnitude.
