抄録
(1) The outersegments of rods are detached from the retinal tissues without damage by crushing the retinae with slides.
(2) The detached outersegments of rods are separated completely by means of a new sugar floatation method.
(3) The process involved in this new sugar floatation method is as follows:
(a) Four liquid layers are prepared in a centrifuge tube. Three of them consist of sugar solutions, of specific gravities 1.200, 1.150, 1.120 respectively and the top layer is made up of distilled water. The pH of them should be 7.0.
(b) Centrifuge at 3000 rpm. for 15 min.
(c) The stratum of floating outersegments at the boundary between two solutions is pipetted off and recentrifuged in a sugar solution of s.g. 1.160.
(4) Gylcerin solutions of various specific gravities can be used as substitutes for the sugar solutions; their specific gravities should be 1.200, 1.180 and 1.150.
(5) Rhodopsin can be extracted from the outersements of rods with various substances hitherto not described.
