1954 Volume 4 Pages 268-273
In dogs anesthetized with evipan-sodium, adrenal venous blood specimenswere collected through the lumbar route without opening the abdominal cavity.
Adrenaline content of the specimens was estimated colorimetrically using the arseno-molybdic acid method of Bloor and Bullen.
After administration of atropine, acetylcholine in a dose of 2-3 mg. per kg.was injected. On receiving acetylcholine the adrenaline secretion rate wasestimated to be markedly augmented. After a while, procaine in a dose of 50-70 mg. per kg. or tetraethylammonium in a dose of 10-20 mg. per kg. was injected.After procaine or tetraethylammonium, acetylcholine in the same doseas before used to cause little or no increase in the rate of adrenaline secretion.