Abstract
The mycelial development of Piricularia Oryzae CAV. is generally poor in òrdinary synthetic nutrient solutions. The authors carried out cultural experiments of this fungus with several synthetic nutrient solutions in an attempt to set up a formula of somewhat more suitable one for the culture of the present fungus. Based upon the results obtained in the cultural experiments the authors contrived the following formula of a synthetic nutrient solution:
Potassium nitrate (KNO3) 2.0g
Monopotassium phosphate (KH2PO4) 0.5g
Dipotassium phosphate (K2HPO4) 0.5g
Magnesium sulphate (MgSO4·7H2O) 0.5g
Calcium chloride (CaCl2) 0.1g
Ferric chloride (FeCl3) trace§
Sucrose 30.0g
Redistilled water 1000.0cc
§ One drop of 5% FeCl3 solution for 30cc of the nutrient solution.
Hydrogen ion concentration of the present solution was about pH 6.3, and a slight precipitation unavoidably took place. The fungus grew tolerably well in this nutrient solution, and a grayish olive colored mycelial colony developed in 30 cc of this solution by a culture in a thermostat at 28°C for 30 days was measured to be more than 4 mg in dry weight. The present formula would be recommended as a suitable synthetic nutrient solution for the mycelial development of Piricularia Oryzae CAV.
