Abstract
The rice-blast fungus was cultivated on the culture media containing either ammonium salt or nitrate as the nitroge source. Between the two myce1ia grown on the above media any marked difference has not been observed in the mycelial constituents, such as reserved carbohydrate, 1ipid and proteinous substance. In both cases rapid absorption of nitrogenous matter and synthesis of protein occured at the initial stage of the growth. On the other hand activity of lipid synthesis which was very low at the initial stage, rose gradually with the growth, The maximum contents of reserved carbohydrate as well as of lipid were observed just before the maximum growth.
Contrary to carbohydrate and lipid, the amount of protein was seen to decrease gradually with the growth, but the relative content increased again owing to exhaustion of carbohydrate and lipid when autolysis began. Activity of exo-and endoamylase of the mycelia grown on these media were almost the same. Though activity of endo-amylase showed little change when the media become acidic by the selective absorption of cation, marked lowering in the activity of exo-enzyme was observed to occur.
