Studies on the chemotherapy for plant virus diseases III. Effect of thiosemicarbazones on the multiplication of tobacco mosaic virus.

Toru SHIMOMURA; Yonosuke NISHIKAWA; Teruo IMAIZUMI

doi:10.3186/jjphytopath.23.65

Studies on the chemotherapy for plant virus diseases

III. Effect of thiosemicarbazones on the multiplication of tobacco mosaic virus.

Toru SHIMOMURA, Yonosuke NISHIKAWA, Teruo IMAIZUMI

Author information

JOURNAL FREE ACCESS

1958 Volume 23 Issue 2 Pages 65-68

DOI https://doi.org/10.3186/jjphytopath.23.65

Details

Published: 1958 Received: - Available on J-STAGE: February 19, 2009 Accepted: February 17, 1958 Advance online publication: - Revised: -
Correction information
Date of correction: February 19, 2009 Reason for correction: - Correction: SUBTITLE Details: Wrong : III. Effect of thiosemicarbazones on the multiplication of tobacco mosaic virus
Right : III. Effect of thiosemicarbazones on the multiplication of tobacco mosaic virus.

Date of correction: February 19, 2009 Reason for correction: - Correction: ABSTRACT Details: Wrong : In the present investigation, inhibitory activity to the multiplication of tobacco mosaic virus (TMV) of thiosemicarbazones from benzaldehyde, acetophenone, cinnamaldehyde, and benzalacetone and the para-substituents of them was tested. Detached tobacco leaves which had been inoculated with TMV 24 hours before by rubbing with a juice of infected tobacco leaf, were split along the midrib. One half of each leaf was floated on solutions of the chemicals in Petri dishes and were kept at 25° under continuous illumination from fluorescent lamps. The another half was kept on water and served as control. After 5∼6 days, the leaves were removed, homogenized, and analyzed for TMV by means of ammonium sulphate-precipitation method previously reported⁷⁾. In some experiments, measurement of the amount of TMV was made by the method of Bancroft and Curtis⁹⁾, and was compared with the results of ammonium sulphate method.
Results of the experiments are given in tables 1∼4. The inhibitory activity of 21 thiosemicarbazones was determined and it was found that both unsubstituted and substituted benzalacetone thiosemicarbazones are effective, to some degree, to the inhibition of TMV multiplication. Benzaldehyde thiosemicarbazones had also some inhibitory activity on the virus, but not the substituted ones. Thiosemicarbazones from acetophenone and cinnamaldehyde with or without para-substituents showed no effect.
Right : In the present investigation, inhibitory activity to the multiplication of tobacco mosaic virus (TMV) of thiosemicarbazones from benzaldehyde, acetophenone, cinnamaldehyde, and benzalacetone and the para-substituents of them was tested. Detached tobacco leaves which had been inoculated with TMV 24 hours before by rubbing with a juice of infected tobacco leaf, were split along the midrib. One half of each leaf was floated on solutions of the chemicals in Petri dishes and were kept at 25° under continuous illumination from fluorescent lamps. The another half was kept on water and served as control. After 5-6 days, the leaves were removed, homogenized, and analyzed for TMV by means of ammonium sulphate-precipitation method previously reported. In some experiments, measurement of the amount of TMV was made by the method of Bancroft and Curtis, and was compared with the results of ammonium sulphate method.
Results of the experiments are given in tables. 1-4. The inhibitory activity of 21 thiosemicarbazones was determined and it was found that both unsubstituted and substituted benzalacetone-thiosemicarbazones are effective, to some degree, to the inhibition of TMV multiplication. Benzaldehyde thiosemicarbazones had also some inhibitory activity on the virus, but not the substituted ones. Thiosemicarbazones from acetophenone and cinnamaldehyde with or without para-substituents showed no effect.

Date of correction: February 19, 2009 Reason for correction: - Correction: DTPUB Details: Wrong : 19580730

Date of correction: February 19, 2009 Reason for correction: - Correction: AUTHOR Details: Wrong : Toru SHIMOMURA, Yonosuke NISHIKAWA, Teruo IMAIZUMI¹⁾
Right : Toru SHIMOMURA¹⁾, Yonosuke NISHIKAWA¹⁾, Teruo IMAIZUMI¹⁾

Date of correction: February 19, 2009 Reason for correction: - Correction: CITATION Details: Wrong : 1) Hamre, D., Brownlee, K. A., & Donovick, R.: J. Immunol., 67: 305. (1951).
2) Thompson, R. L. et al.: J. Immunol., 70: 229. (1953).
3) Minton, S. A. Jr., Officer, J. E., & Thompson, R. L.: J. Immunol., 70: 222. (1953).
4) Schlegel, D. E., & Rawlins, T. E.: J. Bacteriol., 67: 103. (1954).
5) Iwasaki, K., Nishimura, T., Igarashi, Y., & Nagaki, D.: Kitasato Arch. Exp. Med., 28: 31. (1955).
6) Hirai, T., Shimomura, T., & Nishikawa, Y.: Nature (London), 181: 352. (1958).
9) Bancroft, J. B., & Curtis, R. W.: Phytopathology, 47: 79. (1957).
10) Weintraub, M, & Kemp, W. G.: Canad. J. Microbiol., 1: 549. (1955).

Right : 1) Hamre, D., Brownlee, K. A., & Donovick, R.: J. Immunol., 67: 305. (1951).
2) Thompson, R. L. et al: J. Immunol., 70: 229. (1953).
3) Minton, S. A. Jr., Officer, J. E., & Thompson, R. L.: J. Immunol., 70: 222. (1953).
4) Schlegel, D. E., & Rawlins, T. E.: J. Bacteriol., 67: 103. (1954).
5) Iwasaki, K., Nishimura, T., Igarashi, Y., & Nagaki, D.: Kitasato Arch. Exp. Med., 28: 31. (1955).
6) Hirai, T., Shimomura, T., & Nishikawa, Y.: Nature (London), 181: 352. (1958).
9) Bancroft, J. B., & Curtis, R. W.: Phytopathology, 47: 79. (1957).
10) Weintraub, M, & Kemp, W. G.: Canad. J. Microbiol., 1: 549. (1955).

Date of correction: February 19, 2009 Reason for correction: - Correction: PDF FILE Details: -

Abstract

In the present investigation, inhibitory activity to the multiplication of tobacco mosaic virus (TMV) of thiosemicarbazones from benzaldehyde, acetophenone, cinnamaldehyde, and benzalacetone and the para-substituents of them was tested. Detached tobacco leaves which had been inoculated with TMV 24 hours before by rubbing with a juice of infected tobacco leaf, were split along the midrib. One half of each leaf was floated on solutions of the chemicals in Petri dishes and were kept at 25° under continuous illumination from fluorescent lamps. The another half was kept on water and served as control. After 5-6 days, the leaves were removed, homogenized, and analyzed for TMV by means of ammonium sulphate-precipitation method previously reported. In some experiments, measurement of the amount of TMV was made by the method of Bancroft and Curtis, and was compared with the results of ammonium sulphate method.
Results of the experiments are given in tables. 1-4. The inhibitory activity of 21 thiosemicarbazones was determined and it was found that both unsubstituted and substituted benzalacetone-thiosemicarbazones are effective, to some degree, to the inhibition of TMV multiplication. Benzaldehyde thiosemicarbazones had also some inhibitory activity on the virus, but not the substituted ones. Thiosemicarbazones from acetophenone and cinnamaldehyde with or without para-substituents showed no effect.

Corresponding author

Correction information

Register with J-STAGE for free!